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一品红不同外植体愈伤组织诱导能力的研究
A study of induction callus with different organs of Euphorbia pulcherrima Willd
【摘要】 分别用一品红(EuphorbiapulcherrimaWilld)自然植株的叶片、叶柄、休眠芽、顶芽、带皮茎段、去皮茎段,以及经一段时间组织培养的试管苗相应器官作外植体,进行愈伤组织诱导能力的研究.结果表明,在所有试验过的外植体中,以试管苗的幼茎、幼叶为最好,自然植株上所取的幼茎、幼叶次之,愈伤组织诱导率分别为100%和95.0%;叶柄的愈伤组织诱导率极低,为15.0%左右;休眠芽、顶芽均未能诱导成功.
【Abstract】 The callus were inducted on MS medium with leaves,petioles,dormant buds,apical buds,stems as explants respectively.The results indicated that the leaves,stems of testtube plant is the best among explants mentioned above.The induction rates of callus from leaves and stems of testtube plants and of old plants were 100%,95% respectively,with the former being obviously better than the latter,but the rates of callus from petiole carried no significance for either,it is 10%.However,there were no callus induction from such explants as dormant bud and apical bud.
【Key words】 Euphorbia pulcherrima Willd; tissue culture; callus; callus rate; organs;
- 【文献出处】 浙江师范大学学报(自然科学版) ,Journal of Zhejiang Normal University (Natural Sciences) , 编辑部邮箱 ,2003年01期
- 【分类号】S685.23
- 【被引频次】19
- 【下载频次】124