【摘要】 目的　探讨细胞凋亡及Bcl 2、Bax与血管瘤及血管畸形的关系。方法　用SP法测定草莓状血管瘤和血管畸形 6 8例及正常皮肤 11例中Ki 6 7、Bcl 2、Bax的表达 ;用TUNEL法测定内皮细胞凋亡指数 (AI)。结果　Ki 6 7在增生期草莓状血管瘤中的表达阳性率为 10 0 % ,而在其他组中未见阳性表达 ;Bax在草莓状血管瘤中的表达明显高于血管畸形和正常皮肤 ,两者差异有显著性意义 ;Bcl 2均未见阳性表达 ;草莓状血管瘤的AI明显高于血管畸形和正常皮肤 ,差异有显著性意义。在草莓状血管瘤中 ,伴随Bax的表达强度增加 ,AI逐渐增加 ,两者呈正相关。结论　细胞凋亡可能与草莓状血管瘤自然消退有关 ,Bcl 2和Bax在细胞凋亡中可能起着重要的调控作用。更多还原

【Abstract】 Objective To study the expression of apoptosis and their regulatory genes,Bcl-2,Bax in hemangioma and vascular malformations. Methods The specimens were taken from 68 cases of strawberry hemangioma or vascular malformations and 11 cases of normal skin.The expression of Bcl-2,Bax and Ki-67 in endothelial cells were investigated by immunohistochemical S-P method.The apoptosis index(AI)in endothelial cells was investigated by TUNEL method. Results Ki-67 was positive in all the proliferating strawberry hemangioma,negative in the other groups.The expression of Bax was significantly higher in strawberry hemangioma than in vascular malformations and normal skin( P <0.01).Bcl-2 was negative in all groups.The AI in endothelial cells of strawberry hemangioma was significantly higher than that of vascular malformations and normal skin.There were no statstically signficant differences between the proliferating and involution strawberry hemangioma.There were no statistically significant differences between various types of vascular malformations and normal skin.With the increasing of the expression of Bax in strawberry hemangioma,the AI increased. Conclusion These findings clearly demonstrate a much higher apoptotic activity in strawberry hemangioma than in vascular malformations and normal skin and suggest that apoptosis might be a cause of spontaneous involution of strawberry hemangioma and might not be a cause of the pathogenesis of vascular malformation.Bcl-2,Bax might play an important regulatory role in apoptosis in endothelial cells of strawberry hemangioma.更多还原