【摘要】 目的　研究MTS1基因 β启动子在急性T淋巴细胞白血病细胞株中的转录激活情况 ,鉴定 β启动子转录活性的功能片段区。方法　用DNA重组方法 ,构建MTS1基因 β启动子 3′端转录起始点相同 ,而 5′端序列不同的 7种pGL3重组质粒。用脂质体介导的基因瞬时转染法 ,将构建的重组质粒分别转染MTS1基因双等位缺失的Jurkat细胞株 ,检测pGL3重组质粒中荧光素酶报告基因的表达 ,观察 β启动子在Jurkat细胞中的激活情况及其基础转录活性片段区。 结果　成功构建了MTS1基因 β启动子 7种不同片段的重组质粒 ,它们在Jurkat细胞中均有转录活性 ,其中 0 .38kbSacⅡ SacⅠ酶切片段是MTS1基因 β启动子转录活性的基础片段。 结论　MTS1β启动子可在Jurkat细胞中被激活。更多还原

【Abstract】 Objective To investigate the effect of MTS1 gene β promoter transcriptional activation in T cell acute lymphoblastic leukemia(T ALL) cell lines and identify the fragment with transcriptional activation. Methods Seven pGL3 recombinant plasmids with the same 3′ end transcriptional start site but the different 5′sequences were constructed by gene recombinant technique and transfected into Jurkat cell line which is biallelic deletion of MTS1 gene by transient transfection. Luciferase report gene was detected to observe β promoter transcriptional activation. Results Seven pGL3 recombinant plasmids containing different fragments of β promoter were obtained,all of them showed transcriptional activation in Jurkat cell line. Among them, the 0.38 kb fragment cut by SacⅡ SacⅠ is fundamental in transcription. Conclusion MTS1 gene β promoter can be activated in Jurkat cell line.更多还原