【摘要】 目的　多中心临床研究糖基化的G CSF联合中剂量环磷酰胺 (Cy)或增大针对性联合化疗中Cy剂量动员自体外周血造血祖细胞的效果。方法　北京地区 4所医院 30例患者纳入方案。其中非霍奇金淋巴瘤 (NHL) 2 1例 ,霍奇金病 (HD) 1例 ,乳腺癌 7例及卵巢癌 1例。采用中剂量Cy或以增大Cy剂量为基础的针对性化疗联合G CSF动员自体外周血造血祖细胞 (APBPC)。在化疗后白细胞计数达最低值时开始应用G CSF。当白细胞升至 5 .0× 10 9 L以上时 ,用血细胞分离机采集。结果　Cy平均实际使用剂量为 3.95g(2 .3g m2 ) ;G CSF的剂量分别为 2 5 0 μg d(2 9例 ) ,5 0 0 μg d(1例 ) ,实际剂量3 1～ 6 .4 μg·kg- 1 ·d- 1 。 30例患者平均采集 2 .7次 ,其中 13例采集 2次 ,14例采集 3次 ,3例采集 4次。达到目标采集量单个核细胞≥ 6× 10 8 kg为 2 1例 (70 .0 % ) ,CD34 + 细胞≥ 2× 10 6 kg为 30例 (10 0 % ) ,CFU GM≥ 2× 10 5 kg为 2 4例中 15例 (6 2 .5 % )。 1次采集后 2 7例 (90 .0 % )、2次采集后 2 9例 (96 .7% )患者达到了CD34+ 细胞数目标采集量。结论　G CSF 2 5 0 μg d联合中剂量Cy或以增大Cy剂量为基础的针对性化疗可采集到足够数量的APBPC。更多还原

【Abstract】 Objective To investigate the clinical value of glycosylated G CSF combined with middle high dose cyclophosphamide (Cy) or conventional chemotherapy with increased dose of Cy for mobilizing peripheral blood progenitor cells in patients with tumor. Methods Thirty patients from four hospitals in Beijing region were enrolled in this clinical study. Diagnoses of the patients were non Hodgkin’ lymphoma (n=21), Hodgkin disease(n=1), breast cancer (n=7) and ovary cancer (n=1). Autologous peripheral blood progenitor cells (APBPC) were mobilized by middle high dose Cy or conventional chemotherapy with increased dose of Cy combined with G CSF. G CSF was given subcutaneously from the nadir of the white blood cell (WBC) count to the end of PBPC collection. The dosage of G CSF was 250?μg/d in 29 patients and 500 μg/d in 1 patient. When WBC count was > 5×10 9/L, APBPC were harvested with CS 3000 plus/COBE Spectra. Results The average dosage of Cy was 3.95 g(2.3 g/m 2 ). The doses of G CSF were 3.1～6.4?μg·kg -1 ·d -1 . Thirteen patients(43%) were collected twice, 14 patients (47%) three times and 3 patients (10%) four times. All of the patients could tolerate the treatment regimens. Seven patients had bone pain after G CSF injection and one was severe, one patient had headache and one had nausea and vomiting. Conclusion 250 μg glycosylated G CSF combined with middle high Cy or conventional chemotherapy with increased dose of Cy combined G CSF is an optimal method for APBPC mobilization in tumor patients.更多还原