【摘要】 目的　通过大鼠自体平滑肌细胞移植 ,观察其在心肌缺血区的存活情况 ,探讨对心功能的作用。　方法　用酶消化法从SD大鼠的输精管组织中分离、提取平滑肌细胞 ,进行体外培养、扩增 4周。细胞移植组 (10只 )将BrdU标记的自体平滑肌细胞直接注射移植到冠状动脉结扎后 4周形成的心肌瘢痕组织中 ;对照组 (10只 )注射同等剂量的DMEM培养基。多普勒超声检查 ,评估对比移植前和移植后 12周的心室形态以及心功能状况。心脏病理标本用抗平滑肌特异性的α SMA及抗细胞标记物BrdU的单克隆抗体作免疫组化染色 ,检测植入的平滑肌细胞在梗死区的存活情况。　结果　植入的平滑肌细胞 12周后仍存活于心肌梗死区 ,并形成肌样组织。移植组与对照组比较 ,其左室前壁厚度增加〔分别为 (2 36± 0 31)及 (1 0 3± 0 11)mm ,P <0 0 1〕 ,收缩舒张运动协调 ,心室舒张末容积减少〔分别为 (0 94± 0 16 )及 (1 39± 0 17)ml,P <0 0 1〕 ,射血分数提高〔分别为 (4 8 6± 7 2 )及 (2 5 3± 4 1) % ,P <0 0 1〕。　结论　移植的自体平滑肌细胞能长期存活于心肌梗死区中 ,并通过增厚加固心室壁 ,阻止心肌梗死后的心腔扩大和心室重构 ,增加心脏射血 ,有效地提高心功能。更多还原

【Abstract】 Objective The long-term effects of cell transplant on cardiac function are unknown. Therefore, we tested the survival and functional impact of rat autologous smooth muscle cells up to 12 weeks after transplant into infracted hearts. Methods Autologous smooth muscle cells were acquired from the ductus deferens of adult Sprague-Dawley rats(weight,300g), and cultured for 4 weeks before transplant. 4 weeks after left coronary artery ligation, the cultured cells(4×10 6 , n=10), marked with 5-bromo-2’-deoxyuridine(BrdU), or culture medium alone(n=10) were injected directly into infarcts of the heart. Cardiac function was assessed by echocardiography before and 12 weeks after transplantation. BrdU in the cells and smooth muscle cells were identified by immunohistochemical staining technique using monoclonal antibodies against BrdU and α-smooth muscle actin. Results Grafted autologous smooth muscle cells were presented in infarcts and formed musclelike tissue. They thickened the wall of the left ventricle〔(2.36±0.31) vs (1.03±0.11)mm,P<0.01), associated with reduced LV end-diastolic volumes〔(0.94±0.16) vs (1.39±0.17)ml,P<0.01〕 , enhanced LV ejection fraction〔(48.6±7.2) vs (25.3±4.1)%,P<0.01〕, prevented cardiac dilatation and improve ventricular function. Conclusions Autologous smooth muscle cells transplant exhibits long-term survival in a myocardial infarct model and contributes to long-term improved cardiac function. These results suggest that a damaged heart can be rebuilt.更多还原