【摘要】 目的 :建立同时测定葛根中葛根素、大豆苷及大豆苷元的含量测定方法。方法 :选用SYMMETRY C18色谱柱 (3.9mm× 15 0mm ,5 μm) ,甲醇 1%醋酸水溶液为流动相梯度洗脱 ,紫外检测波长为 2 5 0nm ,HPLC法。结果 :平均回收率分别为 10 1.7% ,10 0 .7% ,10 1.7% (n =3) ,RSD分别为 0 .43 % ,0 .82 % ,1.5 0 % (n =3)。结论 :本法简便 ,准确 ,快速 ;适用于含葛根素、大豆苷及大豆苷元的葛根药材及制剂的质量控制。更多还原

【Abstract】 Objective: To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata . Method: The separation was performed in a SYMMETRY C 18 column with a mobile phase of methanol-1%HAC solution (gradient elution), the The detection wavelength being 250 nm. Result: The average recovery was respectively 101.7%,100.7% and 101.7%( n =3),RSD 0.43%, 0.82% and 1.50%( n =3) for Puerarin,Daidzin and Daidzein. Conclusion: This method is suitable for the determination of Puerarin,Daidzin and Daidzein in Pueraria lobata and its preparation.更多还原