【摘要】 目的　建立反相高效液相色谱法测定人脑膜成纤维细胞 (Humanmeninxfibroblast,HMF)的吲哚胺 2 ,3 -双加氧酶 (indoleamine 2 ,3 -dioxygenase,IDO)活性 ,并探讨IDO的临床意义。方法　以荧光检测器和紫外检测器分别检测色氨酸和犬尿氨酸 ,间接测定IDO活性 ,采用3H -尿嘧啶 (3H -U)特异标记的弓形虫在HMF内的增殖实验。结果　色氨酸和犬尿氨酸的平均回收率分别为 99 0 9% ,10 2 18% ,变异系数分别为 1 0 3 % ,1 89% ,最低检测限分别为 8 77,3 2 75ng/ml。被IFN -γ诱导产生的IDO可降解胞内必需氨基酸色氨酸而抑制弓形虫生长。结论　本法为一种灵敏、简便和可靠的反相高效液相色谱法 ,IDO可抑制弓形虫生长更多还原

【Abstract】 Objective To determine indoleamine 2,3-dioxygenase(IDO) activity in human meninx fibroblast(HMF) by using reversed-phase HPLC and establishing a simple and reliable method,and to explore clinical significance of IDO.Methods IDO activity is indirectly determined by detecting the amount of tryptophan and kynurenine with FLD and DAD respectively.The ability to restrict growth of T.gondii was evaluted by 3H-U incroporation assay.Results The average coefficients of recovery of tryptophan an kynurenine were 99.02% and 102.18% respectively,their coefficients of variation were 1.03% and 1.89% respectively,and their minimal detected concentration were 8.77?ng/ml and 32.75?ng/ml respectively.IDO induced by IFN-γ could degrade the intracellular tryptophan to restrict T.gondii growth.Conclusion Determination of IDO activity by using reversed-phase HPLC was a sensitive,simple and reliable method.IDO could restrict T.gondii growth.更多还原