【摘要】 利用激光共聚焦显微技术研究了砂梨 (PyrusserotinaRehd .)品种‘丰水’自花授粉和异花(‘二十世纪’)授粉 16h后花柱自发荧光的分布规律。发现未授粉花柱整体的自发荧光比较弱 ,但从柱头端向子房端花柱自发荧光呈上升趋势 ;异花授粉和自花授粉后花柱整体的自发荧光都有所增加 ;授粉后自发荧光变化最大的部位在柱头区域 ;自花授粉后 ,在柱头及距柱头 0～ 4 0 0 μm范围内荧光值较高 ,其他区域的荧光值相近 ;而异花授粉后在花柱的中部 (距柱头 15 0 0～ 2 0 5 0 μm范围内 )荧光值最低 ;自花授粉后自发荧光除柱头以外主要分布在花柱的边缘区域 ;异花授粉后自发荧光在花柱上半部的分布情况与自花授粉类似 ,下半部分布相对均匀。更多还原

【Abstract】 The variations of style auto fluorescence after self and cross pollination in Pyrus serotina Rehd.were studied with laser scanning confocal microscope (LSCM).The pollination combinations were as follows:’Housui’( S 3S 5 )(♀)×’Housui’(♂) and’Housui’×’Nijiseiki’( S 2S 4 ).The style auto fluorescence increased after pollination and the most remarkable increases occurred in the stigma and the area 0-400 μm down (total length of style is about 3700 μm).After self pollination,with a rapid decline of auto fluorescence from stigma to 950 μm down,there was almost no variation from 950 μm to the end of style.But a visible climbing of auto fluorescence can be detected from 950 μm to the end of style after cross pollination.Results also showed that there were differences of style auto fluorescence distribution between self and cross pollination treatments.All treatments proved that in the upper half of the style the auto fluorescence in the parietal part was stronger than that in the middle part and so did that in the lower part after self pollination.But after cross pollination the auto fluorescence was relatively evenly distributed in the lower half of the style.All above indicated that,with LSCM,visible changes in the style after various pollination treatments can be detected and thus another method to study the self incompatibility in vivo or semi in vivo can be established.更多还原