【摘要】 目的:探讨单唾液酸四己糖神经节苷酯(GM1)对新生大鼠缺氧缺血性脑损伤(HIBI)的治疗作用。方法:选用7 d龄SD大鼠54只,分为假手术组6只(分离但不结扎右侧颈总动脉)、GM1治疗组24只(分离并结扎右侧颈总动脉,置缺氧舱取出后每24 h腹腔注射GM110 mg·kg-1,直至处死)、HIBI组24只(与GM1治疗组同样造成缺血缺氧后,腹腔注射与GM1治疗组同体积0.9％氯化钠溶液),监测不同时间脑组织中超氧化物歧化酶(SOD)、NO、ATP酶水平。结果:与HIBI组相比,GM1治疗组中SOD水平均明显升高,NO水平均明显降低,各种ATP酶均明显增高(P<0.05)。结论:GM1对新生大鼠HIBD有明显的治疗作用。更多还原

【Abstract】 Objective:To probe into the therapeutic effect of monosialoganglioside (GM1 ) in the treatment of hypoxic-ischemic brain injury (HIBI) in neonatal rats. Methods:54 7-day-old SD rats were divided into 3 groups: sham-operating group (6 rats, in which the right common carotid artery was separated but not ligated); GM1 treated group(24 rats, in which the right common carotid artery was separated and ligated. 1/2 hr after the operation, the animals were kept in a hypoxic chamber for 2 hr. These rats were then given each an intraperitoneal injection of 10 mg·kg-1 of GM1 immediately after they had been taken out of the chamber and every 24 hr until they were sacrificed); HIBI group (24 rats, after being rendered ischemic and hypoxic as in rats of the GM, group, animals of this group were given each an intraperitoneal injection of 0.9% sodium chloride solution). The SOD, NO and ATPase levels in the brain tissue were monitored at different time periods. Results: The SOD and ATPase levels in the brain tissue of rats in the GM1 treated group were significantly higher than those of the HIBI group( P < 0.01). The NO level, however, was apparently lower in rats of the GM1 treated group( P < 0.05). Conclusion: GM1 was shown to have a good therapeutic effect in the treatment of HIBI in neonatal rats.更多还原