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猪GH基因部分多态特征与早期体重的相关研究

The Study on the Relationships between Some Polymorphism Restriction Sites of Porcine GH Gene and the Early Bodyweight

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【作者】 陶勇经荣斌任善茂宋成义张金存陈华才

【Author】 TAO Yong1,JING Rongbin2,REN Shanmao1,SONG Chengyi2,ZHANG Jincun3,CHEN Huacai3(1Jiangsu Animal Husbandry and Veterinary College,Taizhou 225300;2.Yangzhou University,Yangzhou 225009;3.Jiangyan Pig Breeding Farm of Jiangsu Province,Jiangyan 225500)

【机构】 江苏省畜牧兽医职业技术学院扬州大学畜牧兽医学院江苏省姜堰市种猪场江苏省姜堰市种猪场 泰州225300扬州225009泰州225300姜堰225500姜堰225500

【摘要】 试验采用PCR RFLP技术,BspⅠ、HhaⅠ两种限制性内切酶,检测到猪生长激素基因-119~+486位共605bp片段中的3处碱基突变位点(+193、+330、+379),分析比较了各多态位点对姜曲海猪早期体重的影响。结果发现:姜曲海猪群中,BspⅠ酶切产生的三种基因型个体的早期平均体重间没有差异,但A1A1基因型个体早期的平均体重略高于其它两种基因型个体。HhaⅠ酶切产生的不同基因型中,C1C2型个体在20日龄、70日龄的平均体重均显著地高于C1C4基因型个体的体重(P<0 05)。

【Abstract】 The genotypes of 73 Jiangquhai pigs at GH gene from -119~+486 were detected by PCRRFLP.The PCR products were cut by BspⅠ and HhaⅠ,and produced three polymorphism sites:one for BspⅠ at +193,two for HhaⅠ at +330 and +379.Effects of different genotypes on early bodyweight were analyzed.The results showed that no significant differences were observed among different BspⅠ genotypes,while 20day and 70day bodyweight of the pigs with C1C2 genotype were significantly higher than that of pigs with C1C4 genotype (P<005).

【基金】 江苏省农业新技术革命项目(DE98503 11)
  • 【文献出处】 畜牧兽医学报 ,Chinese Journal of Animal and Veterinary Sciences , 编辑部邮箱 ,2003年03期
  • 【分类号】S828.2
  • 【被引频次】29
  • 【下载频次】166
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