【摘要】 用DIG标记PS19探针和异硫氰酸荧光素标记的鸡抗IBDVIgG,对人工接种IBDV强毒株Hrb和弱毒细胞适应株Ts后不同时间段的法氏囊、盲肠扁桃体、脾、肾、肝、胸腺、大腿及胸部肌肉的石蜡切片分别进行原位杂交和荧光抗体检测。同时将各时间段每种组织的石蜡切片进行HE染色以观察组织破坏情况。结果发现,用原位杂交和荧光抗体两种方法首先分别于接种Hrb后8h和16h在法氏囊中检测到IBDV的阳性信号,然后依次是盲肠扁桃体、脾、肾、肝、胸腺。侵害程度以法氏囊最重。实验结果表明:Hrb毒株在组织中复制速度快,并迅速在SPF鸡体内传播。Ts毒株侵染较轻,对器官组织无损伤。更多还原

【Abstract】 In situ hybridization (ISH) in which the PS19 probe used was labeled with DIG and directive florescence antibody(DFA)in which the antibody used was labeled with FITC were carried on the bursa,caecal tonsil,spleen,liver,kidney,thymus and thigh muscle infected with Hrb strain and Ts strain at different time post inoculation.These tissues of different time were HEstained to investigate the destroying to these tissues.The result showed that,IBDV could be detected in bursa inoculated with Hrb strain at 8 h(PI)by ISH and 16 h(PI) by DAF.Then signals could be detected in caecal tonsil spleen,liver,kidney,thymus and thigh muscle in turn.Bursa was the mostly infected organ.Compared by Hrb strain,the corresponding tissues infected by Ts strain were less destroyed,and the muscle was uninjured.更多还原