【摘要】 拟定了乳清酸的2.5次微分示波极谱测定新方法。结果表明:在0.8mol/LHCl介质中,乳清酸产生一灵敏的极谱还原波,峰电位Ep=-0.77V(vs.SCE);其2.5次微分极谱峰峰电流ep″与乳清酸浓度在4.0×10-8～4.0×10-5mol/L范围内呈良好线性关系(r=0.9996,n=9),检测限为1.0×10-8mol/L;13次平行测量4.0×10-7mol/L乳清酸的峰电流,所得RSD为1.8%。该方法可直接用于测定牛奶中乳清酸的含量。更多还原

【Abstract】 A 2.5order derivative polarographic method for the determination of orotic acid was proposed.In 0.8 mol/L HCl supporting electrolyte,a reduction wave of orotic acid with peak potential -0.77V (vs.SCE) achieved high sensitivity.The 2.5order derivative peak current of the reduction wave was rectilinear to orotic acid concentration in the range of 4.0×10-8～4.0×10-5 mol/L(r=0.999 6,n=9).The limit of detection was 1.0×10-8 mol/L.Relative standard deviation (RSD) was 1.8% by performing 13 independent measurements for 4.0×10-7 mol/L orotic acid.The proposed method can be applied to direct determination of orotic acid in milk.更多还原