【摘要】 目的　探讨 3种镍化合物诱发细胞恶变过程中不同阶段细胞凋亡情况以及P5 3基因与凋亡之间的关系。方法　 3种镍化合物转化细胞接种BALB c裸鼠 ,应用流式细胞术定量检测转化细胞和肿瘤细胞的凋亡率。结果　在转化的不同阶段 ,随着恶性程度的增加 ,相应细胞系的凋亡率逐渐减少 (P <0 0 1) ,P5 3基因突变细胞凋亡率低于同一阶段未发生突变的细胞 (P <0 0 1)。结论　在该实验条件下 ,镍化合物诱发BALB c 3T3细胞恶性转化的机制中 ,涉及细胞凋亡的受抑 ,P5 3基因的状态在细胞恶变过程中对于凋亡的发生可能起一定的作用。更多还原

【Abstract】 Objective To explore the cell apoptosis and relationship between the cell apoptosis and the P53 gene mutation in the malignant process of BALB/c\|3T3 cells induced by crystalline nickel sulfide,nickel chloride and nickel sulfate.Methods Flow cytometry was used to detect the cell apoptosis of different cells dyed with propidium iodide.Results With the increase of malignant degree,the apoptosis in the cells decreaced gradually in the different stage of malignant process( P <0 01).The cells with P53 gene mutation had a lower percentage of apoptotic cells compared to those without P53 mutation( P <0\^01).Conclusion The mechanism of malignant transforrnation involves the inhibition of apoptosis.The status of P53 gene may play an important role in the induction of apoptosis in the malignant process.更多还原