【摘要】 用固定化方法在 0 .0 5mol/L硫酸介质中制备了血红蛋白 /聚邻苯二胺膜 (Hb/PPD)电极 .所得电极呈现良好的生物催化活性 ,具有典型的酶催化反应动力学特征 .固定化Hb的转移速率常数是Hb的 5 1.9% ,在 4℃下保存 5 0d ,还保持 91.2 %的活性 .研究了不同制备条件对酶电极性能的影响 ,分析了电极上Hb催化反应的动力学 ,探讨了电极电位下Hb的催化活性 ,提出了一种膜与电极之间的电子转移模型更多还原

【Abstract】 A hemoglobin(Hb)/poly-o-phenylenediamine(PPD) membrane electrode was prepared by electropolymerization of o-phenylenediamine on platinum electrode in 0.05 mol/L H 2SO 4 solution. The electrode obtained exhibits significantly well biology catalytic activity and shows kinetic characteristics of the typical enzyme-catalyzed reaction. The transfer rate constant of the immobilized Hb is 51.9% of that of Hb, and it remains the 91.2%activity of the primary after conserved at 4℃ for 50 days. The influence of the electrode preparing condition on the performance of the electrode was studied and the reaction kinetic catalyzed by the Hb on the electrode was analyzed. The catalytic activity of Hb under the electrode potential was discussed and a electron transfer model between the membrane and the electrode was put forward.更多还原