【摘要】 通过对热带小奥德蘑深层发酵培养基碳源、氮源和无机盐的筛选 ,利用正交试验得到了优化发酵培养基配方 ,在此基础上对发酵条件进行了探索。试验结果表明 ,热带小奥德蘑的适宜发酵培养基为 :葡萄糖 3%,蛋白胨 0 .4 %,KH2 PO40 .1%,MgSO4·7H2 O 0 .2 %,VB10 .0 1%。其适宜发酵温度为 2 8℃ ,起始pH5 .0～ 6.0 ,摇瓶装量 60mL 2 5 0mL ,接种量 10 %。更多还原

【Abstract】 The optimum submerged culture medium and culture conditions for Oudemansiella canarii mycelia were studied. The results showed that the composition of the optimum submerged culture medium was glucose 3%,peptone 0.4%, KH 2 PO 40.1%,MgSO 4·7H 2O 0.2% and VB 10.01%, and the optimum submerged culture temperature,initial pH,medium capacity of the shake flask and inoculation rate were 28℃,5.0～6.0,60mL/250mL and 10% respectively.更多还原