【摘要】 了解 TGFβ1 基因对血管平滑肌细胞弹性蛋白表达及与基质黏附力的影响。用 DOTAP脂质体转染p MAMneo TGFβ1 于原代培养的血管平滑肌细胞 ,经 G4 18筛选 ,TGFβ1 表达经免疫荧光鉴定。原位杂交确定弹性蛋白的表达 ,微管吸吮系统确定平滑肌细胞与基质的黏附力。结果表明生理情况下的平滑肌细胞能表达少量的TGFβ1 及少量的原弹性蛋白。经 G4 18筛选 ,外源性 TGFβ1 在血管平滑肌细胞中稳定表达 ,能显著提高 2～ 3倍原弹性蛋白的表达及细胞与基质的黏附力。说明 TGFβ1 在血管组织工程中具有重要的应用价值更多还原

【Abstract】 This study was conducted to examine the effectiveness of a gene transfer of human TGFβ 1 gene into smooth muscle cells and whether the TGFβ 1 can increase elastin expression of smooth muscle cells. With the help of DOTAP, smooth muscle cells were transfected with pMAMneoTGFβ 1.The postive cell clones were selected with G418.The stable transfection and expression of TGFβ 1 in the smooth muscle cells were determined by immunofluorescence analysis.The expression of elastin in the transfected and untransfected cells were determined by in situ hybridization.The adhesion force between smooth muscle cells and matrix was detected by micropipette system.The results shwed abundant TGFβ 1 stable expression in smooth muscle cells. TGFβ 1 gene can increase two three times elastin expression and increase the adhesion between smooth muscle cells and matrix. TGFβ 1 can be used in vascular tissue engineering to increase smooth muscle cells adhesion.更多还原