【摘要】 应用样方法,以三叶草(Trifolium repens)作盆栽扩繁,利用湿筛倾析法和蔗糖离心法分离土壤孢子,研究了广东省常绿阔叶林、人工林(台湾相思林、湿地松林、刚果按林)和木薯地等3种植物群落11个土样的丛枝菌根菌(Arbuscular mycorrhizal fungi,AMF)孢子组成及分布状况。根据孢子形状、颜色、大小、孢壁饰纹及连孢菌丝等形态,将所分离的孢子划分为20个形态型(morphotypes),分属于3科5属。其中球囊霉属(Glomus)有10种形态型,无梗囊霉属(Acaulospora)5种,巨孢囊霉属(Gigaspora)和盾巨孢囊霉属(Scutellospora)各2种,内养囊霉属(Entrophospora)1种。在20个形态型中,以球囊霉属最丰富,分布最广。其中4种类型Glomusdolichosporum(Type I)、Glomus eburneum(Type J)、Glomus versiforme(Type M)和Acaulospora scrobiculata(Type D)在常绿阔叶林、人工林和木薯地中均有出现,其余类型仅见个别土样。更多还原

【Abstract】 The composition of Arbuscular mycorrhizal fungi (AMF) in the three plant communities, evergreen broad-leaf forests, plantations (Acacia confusa, Pinus elliottii, and Eucalyptus leizhou), and cassava croplands in Guangdong Province were surveyed. Trifolium repens was used as a bait plant to trap the AMF spores in the soil sample. The spores were isolated from the soil by sieving and decanting procedure and centrifugation using sucrose. Based on the shape, color, size, wall ornamentation and the structure of subtending hypha, a total of 20 morphotypes of AM fungal spores, belonging to 3 families and 5 genera, were recognized. Of these, 10 types are in Glomus, 5 in Acaulospora, 1 in Gigaspora and Scutellospora respectively and 1 in Entrophospora. Among the 20 spore types, Glomus is the most widespread genus, cf. Glomus versiforme, cf. G. dolichosporum, cf. G. eburneum, and Acaulospora scrobiculata are found in 3 studied communities. The other types are only confined to a few soil samples.更多还原