【摘要】 建立了未衍生化高效液相色谱法(HPLC)测定茶叶中茶氨酸含量的方法。采用的色谱条件为:C18色谱柱,以0 05%(体积分数)三氟乙酸水溶液为流动相,流速1mL/min,进样量10μL,检测波长203nm。茶氨酸质量浓度在0 02～1g/L内,其浓度与峰面积呈良好的线性关系,最低检出限为1 75ng(S/N=3),回收率为97 2%,相对标准偏差(RSD)为1 7%。同时以高效液相色谱 电喷雾离子化质谱对所分离的茶氨酸进行了纯度鉴定。方法具有精确、灵敏、流动相组成简单等特点。更多还原

【Abstract】 High performance liquid chromatography (HPLC) coupled wiht electro-spray ioniza tion mass spectrometry (ESI-MS) was used for the determination and identificat ion of theanine in tea without derivatization. A reversed-phase HPLC method was developed and the effects of the mobile phase composition on separation were investigated. Theanine can be separated completely from other components on a Sp herigel C 18 column with trifluoroacetic acid solution (pH 3 0) as mobile phase. The UV detection wavelength was set at 203 nm. The limit of detection wa s down to 1 75 ng. The method is simple and accurate.更多还原