【摘要】 本文提出了一种测定肉中粗氨的新方法,可用于肉品新鲜度的检验。在常温下,NH3在亚硝基铁氰化钠的催化下于碱性介质中与次氯酸钠和水杨酸作用,生成稳定的蓝色化合物,最大吸收波长为660nm,表观摩尔吸光系数ε660为1.84×104L/mol·cm,Sandell灵敏度为0.001μg/cm2,回归方程为:y=0.108x+0.0022线性范围为:0～0.5μg/ml,相关系数为0.9999,回收率在96%～103.3%之间。更多还原

【Abstract】 A new method for the determination of the crude ammonia in meat was presented in this paper.The method was basedon the color reaction of ammonia-salicylic acid-sodium hypochlorite with the presence of sodium nitroprusside in basic medium.It showed that the apparent molar absorptivity was 1.84×104L/mol·cm with the wavelength at 660nm.Beer’s law was obeyedfor the nitrogen of ammonia in the range of 0～5μg/10ml. The correlation coefficient is 0.9999. The recoveries were between96%～103.3%.The method has been applied satisfactorily to the determination of the crude ammonia in meat samples.更多还原