【摘要】 目的 :探讨加温对乏氧培养中的人舌癌Tca8113细胞株增殖活性的影响 ,为临床上加温治疗口腔癌提供理论基础。方法 :根据是否给予加温处理分为A组 (未加温组 )和B组 (加温组 ) ;根据乏氧时间A、B组又分别分为 0h、3h、6h、12h、2 4h 5个小组。依此分组 ,采用MTT法检测加温对乏氧人舌癌Tca8113细胞增殖活性的抑制率。结果 :加温可降低乏氧人舌癌Tca8113细胞的增殖活性 ;且随着乏氧时间的延长 ,此作用越明显。结论 :乏氧可提高肿瘤细胞对加温的敏感性 ,为加温联合放、化疗治疗口腔癌提供进一步的理论依据更多还原

【Abstract】 Objective: To observe the effect of hyperthermia on the proliferative activity of hypoxic human tongue squamous cell carcinoma(Tca8113) cell-lines,so as to support theories for the clinic application of hyperthermia to treat oral squamous cell carcinoma.Methods:We divided the specimens into 2 groups,group A without hyperthermia;group B with hyperthermia(43℃,40 min) Then according to the different hours of hypoxia,both group A and group B were divided into 5 groups as follows:0h group?3h group?6h group?12h group?24h group,respectively.The proliferative activity of hypoxic Tca8113 cell was examined using MTT essay.The data was statistically calculated by analysis of variance.Results:Hyperthermia could inhibit the proliferative activity of hypoxic Tca8113 cells.This inhibitory effect increased with the increasing hours of hypoxia.Conclusion:Hyperthermia can inhibit the proliferation of oral squamous carcinoma cells and under the hypoxic condition the inhibitory effect was important.It supports the theory of clinic application of hyperthermia or hyperthermia combined with radiotherapy/chemotherapy for oral squamous cell carcinoma.更多还原