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一种竹寄生真菌的分离鉴定及其有效成分发酵的初步研究

Separation and Evaluation of a Bamboo Parasitic Fungus and Studies on Solid-State Fermentation Technology of Its Producing Perylenequinones

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【作者】 李达旭赵建何颖杨志荣

【Author】 LI Daxu, ZHAO Jian,HE Ying YANG Zhirong(Natural Lab. of Biocontrol Engineering of Grassland Pests, Sichuan University, Chengdu 610064,China)

【机构】 四川大学国家草原生物防治工程专业实验室四川大学国家草原生物防治工程专业实验室 成都610064成都610064成都610064

【摘要】 从乐山峨边县采集了一种竹寄生真菌,经过形态比较、菌丝特征的显微观察、组织切片和孢子的显微观察,以及其有效成分的比较,鉴定为竹黄(shiraiabambusicola).其菌丝分离纯化后,以大米及马铃薯汁为基础培养基,分别研究了温度,pH值,碳源,氮源,水份等对该菌在固态条件下产艹北醌化合物的影响.并用正交实验选择了固态发酵的最佳培养条件:每kg基础培养基(大米)中添加硝酸铵20g、麦芽糖50g和马铃薯汁1200mL,温度26℃,自然pH值下,每克菌丝艹北醌类化合物的产量可达到2.4色价.

【Abstract】 A Parasitic fungus was collected from Leshan, Sichuan province and evaluated as shiraiabambusicola by comparing its form, observing hypha characteristics and tissue section and spore in the Microscope and comparing its utility component . After separating its hypha, Potato juice and rice being used as basic medium, the effects of incubation temperature, pH value of the medium, sources of Carbon and Nitrogen , and water on the fact which this fungus produced perylenequinones in the solid state condition, and the optimum culture condition of the solidstate fermenation was obtained by using erthoexperimental design of L9(34 ): 20 g nitric ammonium, 50g malt sugar and 1200ml Potato juice being added to 1 kg basic medium, temperature 26℃, pH natural. The total perylenequinones of its 1g hypha was about 2.4 chromovalue.

  • 【文献出处】 四川大学学报(自然科学版) ,Journal of Sichuan University (Natural Science Edition) , 编辑部邮箱 ,2003年01期
  • 【分类号】Q949.32
  • 【被引频次】22
  • 【下载频次】221
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