【摘要】 [目的]研究通痹灵(TBL)的有效部位总生物碱对细胞增殖及T淋巴细胞转铁蛋白受体(CD71)的影响,探讨通痹灵调节细胞免疫的作用机理。[方法]培养大鼠淋巴细胞,刀豆蛋白(ConA)刺激72 h,四甲基偶氮唑盐(MTT)法检测通痹灵总碱对淋巴细胞增殖的影响;佛波醇酯(PDB)或ConA刺激48 h,流式细胞术检测T淋巴细胞CD71的表达率。[结果]不同浓度的TBL总碱可明显抑制ConA刺激下的淋巴细胞增殖;不同浓度的TBL总碱对PDB和ConA激活的T淋巴细胞CD71表达均有明显的下调作用,而且呈明显的量效关系,对ConA刺激下的CD71表达抑制要强于PDB。[结论]TBL总碱可抑制异常增殖的T淋巴细胞,其作用机制可能是通过抑制转铁蛋白受体来实现的。更多还原

【Abstract】 [Objective]To observe the effects of total alkaloids (TA) of Tongbiling (TBL) prescription on proliferation of T lymphocytes and transferring receptor of T lymphocytes (CD71) in rats and to explore the mechanism of TBL in regulating cellular immunity. [Methods]Isolated lymphocytes from mesenteric lymph node were cultured with various concentrations of TBL TA and canavaline A (ConA) for 72 hours; the proliferation of T lymphocytes was observed by MTT method and CD71 expression rate of T lymphocyte activated by phorbol 12,13-dibutyrate (PDB) or ConA for 48 hours was detected by flow cytometry.[Results]Various concentrations of TBL TA inhibited the proliferation of T lymphocytes activated by ConA and decreased CD71 expression rate of T lymphocyte activated by ConA or PDB in a dose-effect manner. Inhibitory effect of TBL TA on CD71 expression rate of T lymphocyte activated by ConA was superior to that activated by PDB. [Conclusion]TBL TA can inhibit the abnormal proliferation of T lymphocytes and its mechanism may be related to the expression of CD71.更多还原