【摘要】 利用聚丙烯酰胺凝胶电泳技术,对分别来自山东日照和福建厦门两自然群体的沿海花鲈,分析了酶表达活性强的肝脏、肌肉两组织中LDH、MDH、ME、α-GPDH、ADH、SDH、SOD、G-6PDH、EST、GDH等10种同工酶,检出19个基因座位。数据处理结果:花鲈两群体的遗传相似度和遗传距离分别为0.9902和0.0098,平均杂合度分别为0.1112及0.0939。Me-1、G-6-pdh-2、Est-1、Est-5的4个基因座位具有多态性,多态座位比例均为21.1%,座位平均有效基因数均为1.263。由此可表明日照花鲈群体的遗传多样性水平比厦门花鲈群体高。更多还原

【Abstract】 The Sea Bass(Lateolabrax japonicus),captured from the offing of Xiamen F ujian province and Rizhao Shandong province,were samp led for the study.Polyacrylamide gel electrophoresis was used to detect the expression of isozymes in muscle and liver,in which the major isozymes are expressed well and strong-ly.Altogether,10isozymes includi ng LDH?MDH?ME?α-GPDH?ADH?SDH?G -6-PDH?EST?GDH were an-alyzed in 3electrophoresis buffer s ystems.The results of the allelic fr equencies of 19loci showed that the Genetic Similarity and Genetic Distance between two stocks are 0.9902and 0.0098respectively.Average heterozygosity of Rizhao stock was 0.1112,and Xiame n stock was 0.0939.Notably,polymorphic loci of two stocks were both detected in the loci of Me -1?G -6-pdh -2?Est -1and Est -5.The mean number of alleles per locus and the percentage of polymorphic loci measured in two stocks were 1.263and 21.1%.Rizhao stock holds higher degree of polymorphism than Xiamen stocks’.更多还原