【摘要】 目的 :观察鲑鱼降钙素 (sCT)在体外对人乳腺癌细胞生长的影响。方法 :体外培养人乳腺癌细胞系MCF 7,细胞计数法观察sCT对细胞生长的影响 ,透射电镜和DNA电泳法观察凋亡 ,流式细胞仪检测细胞周期和凋亡率。结果 :sCT实验组乳腺癌细胞的数量较对照组减少 (P <0 .0 5 ) ,透射电镜观察到凋亡小体 ,DNA电泳出现梯状条带 ,细胞凋亡率由 3 .72 %升高至 2 3 .3 7% ,G2 期细胞比例由0 .88%升高至 4.13 %。结论 :sCT体外能抑制人乳腺癌细胞生长 ,诱导乳腺癌细胞凋亡和调节细胞周期更多还原

【Abstract】 Objective:To explore the effects of Salmon Calcitonin (sCT)on the growth of human breast cancer cell line.Methods:Human breast cancer cell line MCF-7 was cultivated in vitro.The effects of sCT on cell growth was observed by cell number counting.Transmission electron microscope and DNA electrophoresis were used to observe apoptosis.Cell cycle distribution and apoptosis rates of MCF-7 cells were detected using flow cytometry.Results:The cell number was decreased in sCT treated group(p<0.05).Apotosis body was observed by using transmission electron microscope.DNA ladder was detected in electrophoresis.Cell apoptosis rate increased from 3.72% to 23.37% and G 2 phase rate increased from 0.88% to 4.13%.Conclusion:sCT could inhibit human breast cancer cells by inducing apoptosis as well as regulating cell cycle distribution.更多还原