【摘要】 桑树(MorusalbaL.)变异株系凤尾一之濑是新一之濑的辐射突变体,叶片细长,横向皱缩。利用cDNA-AFLP技术对凤尾一之濑和新一之濑的顶芽进行基因表达差异分析,得到大约3200个条带,20个条带存在差异表达,对其中的15个差异条带进行了克隆和测序。序列同源性检索显示,编号为J12的片段与脯氨酰4-羟化酶α亚基基因有较高的同源性,其余片段与现有的网上基因资源相比没有同源性。实验建立了一套利用桑树变异株系寻找差异表达基因的技术体系。更多还原

【Abstract】 Fengwei-Ichinose is a leaf shape mutant derived from mulberry (Morus alba L.) variety Shinichinose, with narrow and transverse crimple leaves. To identify the differentially expressed genes that respond for the change of leaf shape in this mulberry mutant, a cDNA-AFLP (cDNA-amplified fragment length polymorphisms) analysis has been performed to screen the differentially expressed transcripts in their apical buds. Among 3 200 detected bands, 20 bands showed difference at the expression level. Of the 15 cloned fragments, only one showed homology with a known gene that encodes prolyl 4-hydroxylase alpha subunit, and the other 14 fragments were found no homology with any known genes. This results suggested that the expression of concerned genes might relate to the change of the leaf shape in the mulberry mutant. In this study, the technique system was established for screening the differentially expressional genes between mulberry mutants and their original types.更多还原