【摘要】 利用cDNA-AFLP差异显示技术比较了棉花(Gossypiumhirsutum L.)品系徐州142及其无纤维突变体的胚珠在纤维起始期(开花后0和4d)的基因表达,结果表明,在总共显示的561条带中两种胚珠各有特异带83条。将正常有纤维胚珠的差异片段47个进行反向Northernblot检验,32个在有纤维胚珠中特异表达。选取18个在有纤维胚珠中特异表达的片段进行序列分析,结果表明,8个分别与polyphosphoinositide结合蛋白、细胞色素氧化酶亚基Ⅱ、β-葡萄糖苷酶、蔗糖合成酶、Ca2+通道蛋白、水稻高渗反应蛋白osr40、类TSO1蛋白序列有相似性;4个与拟南芥(Arabidopsisthiliana )的假拟蛋白相似;另有6个找不到相似序列。更多还原

【Abstract】 Using cDNA-AFLP technique, gene expressions in the ovules of cotton (Gossypium hirsutum L.) Xuzhou142 (WT) and its lint and fuzz and mutant (fl ) at fiber initiation stage(0 and 4 d post anthesis) were compared. The result showed that among 561 amplified bands, 166 belonged to two kinds of ovules, of which 83 specially displayed in WT ovules and 83 in fl , respectively. Using cDNAs as probe, 47 WT special fragments were further analyzed by reverse Northern blot, of which 32 were found to be differentially expressed in the WT ovule, and presumably related to the fiber initiation. Sequence analysis demonstrated that, among 18 sequenced fragments, eight were sequence-similar to proteins in GenBank, i.e. polyphosphoinositide binding protein, cytochrome oxidase subunit Ⅱ, beta-glucosidase, sucrose synthase, calcium channel alpha-1D subunit, rice osmotic response protein osr40 and TSO1-like protein, four were similar to hypothetical proteins of Arabidopsis thiliana , and no similar sequence were found in GenBank for the other 6 fragments.更多还原