【摘要】 为探讨万寿菊根氯仿提取物 (TPC)在光照和非光照条件下对山楂叶螨酯酶活性的影响 ,利用垂直板聚丙烯酰胺凝胶电泳法对山楂叶螨酯酶同工酶进行测定。凝胶成像扫描分析结果表明 :随TPC处理浓度增大 ,同工酶带数量减少、扫描曲线OD值降低及峰面积减小 ,其中光照处理对酯酶的抑制程度显著高于非光照处理 ,且所有提取物处理组均无新酶带出现 ,说明万寿菊根氯仿提取物对山楂叶螨酯酶具有较高的光活化活性 ,表现为对酯酶活性的抑制作用更多还原

【Abstract】 The activities of esterase isoenzyme in Tetranychus viennensis Zacher were tested with Polyacrylamide gel electrophoresis after photo and dark reactivation treated by the chloroform extracts, which was obtained by sohxlet method from the root of Tateges erecta. The results showed that both photo and dark treated group with the chloroform extracts could inhibit the activities of esterase isoenzyme. With the increase in the concentration of the extracts, the number of the isoenzyme lanes, the OD value and the peaks area of the gel’s scanning curves reduced gradually. The inhibition potential of photo treated group was obviously significant higher than that of the dark treated group. Neither light group nor dark group, there was not any new isoenzyme band coming out after treated by the extracts. Thus, it seems to be that the chloroform extracts of the root of Tateges erecta have some inhibiting activities to the esterase isoenzyme in Tetranychus viennensis Zacher after treated with light.更多还原