【摘要】 目的　研究中药复方重肌灵对实验性自身免疫性重症肌无力 (EAMG)大鼠免疫调节作用。方法　建立Lewis大鼠EAMG模型 ,将动物分为正常组、佐剂组、模型组、重肌灵组。分别采用 3H TdR掺入法、ELISA法 ,从N2 AchR对淋巴结细胞特异性增殖反应、血清中AchRab滴度、血清IL 4、IFN γ水平等方面观察重肌灵对EAMG大鼠免疫功能的调节作用。结果　与模型组相比 ,重肌灵组可使N2 AchR所致的淋巴结细胞增殖反应受到抑制 (P <0 0 1) ;血清AchRab滴度明显降低 (P <0 0 1) ;血清IL 4、IFN γ水平下调 (P <0 0 1)。结论　重肌灵可能是通过下调血清IL 4、IFN γ ,抑制淋巴结细胞特异性增殖反应 ,抑制AchRab产生而起到治疗作用更多还原

【Abstract】 Objective To study the immunoregulatory effects of Zhongjiling (ZJL), a TCM compound preparation, in the rats with experimental autoimmune myasthenia gravis (EAMG). Methods Lewis rat was used to establish the EAMG model. There were 4 rat groups in the experiment, namely the normal control, CFA, model, and ZJL. The immunoregulatory effects of ZJL on EAMG were observed through detecting the changes in the N 2AchR-induced specific proliferation reaction of the lymphocytes in lymph nodes, serum AchRab titer, and the levels of serum IL-4 and IFN-γby JH3,D,Z-TdR permeation method and ELISA. Results In the ZJL-group, as compared with the model group, the N 2AchR-induced specific proliferation reaction of the lymphocytes in lymph nodes was inhibited (P<0.01), the serum AchRab titer was lowered (P<0.01), and the levels of serum IL-4 and IFN-γ were downregulated (P<0.01). Conclusion The mechanism of the immunoregulatory effects of ZJL may be downregulating the levels of serum IL-4 and IFN-γ, inhibiting the specific proliferation reaction of the lymphocytes in lymph nodes, and restraining the production of AchRab.更多还原