【摘要】 为配合山羊乳腺生物反应器的研究,构建定点整合“打靶框架”和筛选同源基因,用SuperCosl建立了山羊基因组粘粒文库。本试验共获得3.62×10~5个克隆,阳性克隆率达到97.5％,插入片段大小为34～45 kb,平均为40 kb。用5对引物分别对文库做PCR分析,均有特异性扩增。更多还原

【Abstract】 To cope with the research of transgenic goat bioreactor and the construction of a targeting frame, goat ge-nomic library was constructed by using SuperCosl cosmid carrier. 3. 62 × 105recombinants were collected, their average size was 40 kb, the proportion of positive clones was about 97. 5% . The library was screened with 5 pair of primers, the results showed that they were tested positive.更多还原