【摘要】 目的　研究压力对大鼠颈总动脉血管平滑肌细胞整合素 β3、桩蛋白和纽蛋白的分布与表达的影响 ,探讨它们在血管平滑肌细胞的机械应力信号转导中的作用。　方法　不同压力 (16 0mmHg和 80mmHg)灌流大鼠颈总动脉 30min ,免疫组织化学和免疫电镜观察整合素 β3、桩蛋白和纽蛋白在血管平滑肌细胞上的表达和分布变化。　结果　高压和常压灌流对大鼠颈总动脉平滑肌细胞的整合素 β3、桩蛋白和纽蛋白的表达无明显影响 ,但高压使整合素β3、桩蛋白和纽蛋白在平滑肌细胞膜上的分布发生改变 ,向离管腔面的平滑肌细胞膜聚集。　结论　高压诱导了整合素 β3、桩蛋白和纽蛋白向离管腔面的平滑肌细胞膜聚集。提示整合素 β3、桩蛋白和纽蛋白可能在血管平滑肌细胞的机械应力信号转导中发挥作用更多还原

【Abstract】 Objective To investigate the effect of pressure on distribution and expression of integrin β 3,paxillin and vinculin in vascular smooth muscle cells of carotid common artery of rats. Methods The arteries were perfused by both high(160? mmHg) and control(80?mmHg) pressure for 30?min.Distribution and expression of integrin β 3,paxillin and vinculin were studied by immonohistochemistry and immunohisto\|electromicroscopy. Results The expressions of integrin β 3,paxillin and vinculin were no significant difference between high and control pressure groups,but more immunopositive particules accumulated on the ablumen membrane of VSMCs.Conclusion\ The distributions of integrin β 3,paxillin and vinculin are changed in responses to high pressure,which imply that the integrin β 3,paxillin and vinculin may play a role in the mechanotransduction of VSMCs.\;[更多还原