【摘要】 目的　观察人类甲状腺癌中neu癌基因 p1 85蛋白和mRNA的表达及其关系。方法　应用免疫组化和同位素点杂交技术对新鲜冰冻甲状腺组织Neu癌基因p1 85蛋白和mRNA的表达进行研究。 结果　 2 6例甲状腺乳头状癌中 1 7例 p1 85癌蛋白表达阳性 ,而其他类型肿瘤和非肿瘤组织均未见 p1 85表达。Neu癌基因mRNA表达研究显示 ,乳头状癌和淋巴结转移灶中mRNA的表达水平明显高于其他肿瘤和非肿瘤组织。本研究还证明石蜡包埋的乳头状癌标本中Neu癌基因蛋白表达率明显低于新鲜标本。结论　Neu癌基因p1 85蛋白的表达是甲状腺乳头状癌的一个特征性改变 ,Neu蛋白的表达与mRNA的表达相一致 ,Neu癌蛋白检测的稳定性取决于组织标本的质量更多还原

【Abstract】 Objective To investigate the expression and relationship of p185 oncoprotein and mRNA of Neu oncogene in human thyroid carcinomas.Methods p185 oncoprotein and mRNA of Neu oncogene were detected in frozen thyroid tissue specimens using immunohistochemistry and isotope Dot blot hybridisation.Results p185 immunoreactivity was detdcted in17 out of 26 papillary carcinomas,while no Neu oncoprotein immunstaining was seen in cases of the other thyroid tumors and non-neoplastic tissue.Neu mRNA was analysed by Dot blot hybridisation.Papillary carcinomas and lymph node metastases showed significantly higher levels of Neu mRNA compared to the other thyroid tumors and non-neoplastic tissue.The positive rate of Neu oncoprotein on formalin-fixed,paraffin-embedded material were much lower than levels on frozen tissue.Conclusion The present work presents p185 oncoprotein expression of Neu oncogene as a feature of papillary thyroid carcinomas,and is consistent with mRNA expression.The detecting stability of Neu oncoprotein is depand on optimal tissue quality.更多还原