【摘要】 目的 :探讨氨酪酸 (GABA)与空肠上皮细胞分化增生之间的关系。　方法 :用免疫荧光及激光共聚焦显微技术 ,对GABA及谷氨酸脱羧酶 (GAD ,包括两种异构体GAD6 5及GAD6 7)在大鼠空肠上皮中的表达进行研究。另以麦胚凝聚素组织化学染色与GAD6 5免疫荧光结合进行双重染色 ,显示GAD6 5表达细胞的分布特征。同时 ,用增生细胞核抗原 (PCNA)免疫组化方法显示上皮细胞的增生带。　结果 :GABA及GAD6 5阳性细胞主要分布在空肠绒毛的顶端 ,空肠上皮细胞GAD6 7阴性。双重染色显示 ,杯状细胞不表达GAD6 5。PCNA免疫染色阳性细胞主要在隐窝的中下部。表明GABA及GAD6 5主要分布在空肠上皮的成熟带及功能带。　结论 :GABA系统可能与上皮细胞的成熟及分化有关。更多还原

【Abstract】 Objevtives: To explore the relationship between γ aminobutyric acid (GABA) and the proliferation or differentiation of jejunal epithelial cells. Methods: Immunohistochemical expression of GABA, glutamate decarboxylase (GAD, including two isoforms, GAD65 and GAD67) was investigated in rat jejunum. Meanwhile, double staining was performed with GAD65 immunohistochemistry followed by lectin histochemistry of fluorescent wheat germ agglutinin. Furthermore, cell kinetics was evaluated by immunohistochemistry using a monoclonal antibody to proliferating cell nuclear antigen (PCNA). Results: Immunoreactive GABA and GAD65 were distributed in the villi of jejunum, while GAD67 was negative in epithelial cells. Strong positive staining of GABA and GAD65 was mainly localized along the brush border of jejunal villi. In addition, there existed a few GABA and GAD65 strong positive cells scattering in upper portion of the jejunal villi. Double staining showed that immunoreactive GAD65 was not found in goblet cells. PCNA staining nucleoli were found in the lower and the middle portions of the jejunal crypts. Therefore, GABA and GAD65 were expressed in maturation or functional zone. Conclusion: It suggests that GABA might be involved in the regulation of differentiation and maturation of epithelial cells in rat jejunum.更多还原