【摘要】 目的 :研究急性脊髓损伤后神经细胞凋亡的分布特点及其意义。　方法 :Wistar雌性大白鼠 5 4只 ,使用改良Allen法制作急性脊髓损伤模型 ,分别于术后 1、4、8、2 4、72h及 7、14、2 1天处死取材。应用苏木精 伊红染色及凋亡细胞原位末端标记 (TUNEL)法对脊髓组织进行标记。　结果 :损伤后 4h ,在损伤段及邻近段可见末端标记的阳性神经细胞 ,损伤段灰质中阳性细胞数 2 4h达高峰 ,白质中阳性胶质细胞数量 72h达高峰。相邻节段阳性细胞数量在 72h达高峰。灰质中神经元及胶质细胞均有阳性表达 ,但以胶质细胞为主。　结论 :脊髓损伤后神经细胞凋亡是继发损伤期的重要病理变化 ,并有其时相和空间分布特点更多还原

【Abstract】 Objectives: To study the characteristics of neural cells with apoptosis after acute spinal cord injury. Methods: The spinal cord injury of the Wistar rats was induced with Allens way by a 10 g× 2.5 cm impact on the posterior T 8, 9 spinal cord. Rats were sacrificed at 1,4,8,24,72 hours and 7,14,21 days after injury( n =6 for each time point). Three segments of every spinal cord were cut for morphological studies, including hematoxylin and eosin staining and the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) methods. Results: TUNEL positive cells were found in the compression region and neighbor regions after 4 h, with a maximum presence at 24 h in the compressed segment, and labeled glial cells in white matter reached a peak at 72 h. But the labeled cells in the neighboring segments reached a peak at 72 h after injury. The TUNEL positive cells were neural and glial cells in gray matter, especially in the latter one. Conclusions: Apoptosis of neural cells is an important morphological change in the secondary lesion period after spinal cord injury.更多还原