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Interaction of zinc finger ZNF191(243—368) and its I323W and R327W mutants with oligonucleotides by florescence spectrum

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【Author】 ZHOU Jing 1, 2, LIU Yuqi 1, TANG Liming 1, TENG Xinchen 1, TANG Ning2 & HUANG Zhongxian1 1. Department of Chemistry, Fudan University, Shanghai 200433, China; 2. School of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China Correspondence should be addressed to Huang Zhongxian (email: zxhuang@fudan.edu.cn)

【机构】 Department of Chemistry, Fudan University, Shanghai 200433, China； School of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China,Department of Chemistry, Fudan University, Shanghai 200433, China,Department of Chemistry, Fudan University, Shanghai 200433, China,Department of Chemistry, Fudan University, Shanghai 200433, China,School of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China,Department of Chemistry, Fudan University, Shanghai 200433, China；

【Abstract】 Two mutants of the zinc finger protein, ZNF191(243—368) I323W and R327W, are suc-cessfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants and the oligonucleotides. The influence of the mutation on the interaction has been studied using ethidium bromide (EB) as the fluorescence probe. The binding constants of the I323W-DNA and R327W-DNA have been calculated and the possible binding models have been discussed.更多还原

【关键词】 zinc finger protein ZNF191(243—368)； site-directed mutagenesis； fluorescence spectrum； protein-DNA inter-action.；
【Key words】 zinc finger protein ZNF191(243—368)； site-directed mutagenesis； fluorescence spectrum； protein-DNA inter-action.；

【基金】 This work was supported by the National Natural Science Foundation of China (Grant Nos. 30170228 and 39990600).

【文献出处】 Science in China(Series B) ,中国科学B辑(英文版) , 编辑部邮箱 ,2003年01期
【分类号】Q51
【下载频次】19

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