【摘要】 铂丝电极(长0.8cm,直径100μm)经铂化处理后,将3位取代的吡咯衍生物(3-羰基丁酸吡咯)与单体吡咯以适当的比例混合,与葡萄糖氧化酶(GOD)一起用循环伏安法电聚合到铂化的铂丝电极上,形成杂聚吡咯-GOD膜,制备成葡萄糖微酶电极。该微酶电极灵敏度高(每毫摩尔葡萄糖电流响应可以达到700nA),响应范围大(0.1～110mmol/L),抗干扰(抗坏血酸在该电极上的响应电流不到对比电极(无膜)的1.0%),电极的稳定性良好,间断测定40d,灵敏度变化不大。更多还原

【Abstract】 Glucose oxidase was entraped onto a platinized platinum wire (0.8 cm length, 100 μm) by heteropolypyrrole film, which was formed by electropolymerizing a mixture of glucose oxidase, pyrrole and a substituted pyrrole, 4(3pyrrolyl)4oxobutyric acid. The microenzyme electrode showed high sensitivity (up to 700 nA/(mmol·L-1)), broad response range (at 0.1～110 mmol/L of glucose concentration), long stability and no interference by ascorbic acid (at 0.1 mmol/L).更多还原