【摘要】 研究了在富铬酵母的制备过程中加入稀土化合物La2 O3 提高有机铬含量的方法 .实验结果表明 :La2 O3 对酵母同化无机铬为有机铬有明显的促进作用 ,La2 O3 能显著提高富铬酵母中总铬和有机铬的含量而降低 6价无机铬的含量 .当培养基中添加 10 0 μg·g-1La2 O3 时 ,富铬酵母中总铬的含量可达到70 8 33μg·g-1,有机铬的含量可达到 6 39 0 μg·g-1,约为未加La2 O3 的富铬酵母样品的 3倍 ;而 6价无机铬占总铬的质量分数却由 1 6 0 %降至 0 72 % .首次报道了富铬酵母中有机铬的紫外光谱和红外光谱特征吸收峰 ,富铬酵母在 2 6 0nm和 4 78cm-1处有特征吸收峰 ,而加La2 O3 的富铬酵母样品在此两处的吸收峰值均显著增强 ,表明La2 O3 的加入有利于富铬酵母中有机铬含量和葡萄糖耐量因子含量的增加更多还原

【Abstract】 La 2O 3 is added into the culture medium to increase the organic chromium content and the nutritional level of chromium enriched yeast. Experimental results showed that La 2O 3 played an important role in assimilating inorganic chromium to organic chromium, and La 2O 3 could increase the content of total chromium and organic chromium and lower the content of inorganic chromium(VI). When 100 μg·g -1 La 2O 3 was added into the culture medium, the content of total chromium and organic chromium in the cultured chromium enriched yeast was 708 33 μg·g -1 and 639 0 μg·g -1 respectively, which was as three times as that of chromium enriched yeast without La 2O 3 in the culture medium, and the inorganic chromium(VI) was lowered from 1 60% to 0 72%.The UV and IR spectra of the cultured chromium enriched yeast were measured, it was found that there were absorption peaks of organic chromium at 260 nm and 478 cm -1 , and the absorption peaks of the sample added La 2O 3 was higher. The study results proved that the content of organic chromium and glucose tolerance factor in the cultured enriched chromium yeast was increased.更多还原