【摘要】 目的 :并行检测原发性肝癌 p5 3基因突变类型。方法 :参照国际公共 p5 3突变数据库资料发生频率最高的 7个突变类型作为检测探针设计寡核苷酸芯片 ,应用该项技术检测肝癌抑癌基因 p5 3上对应的 7个常见突变位点的突变频率及形式。结果 :检测高发区及低发区肝癌石蜡包埋标本各 14例 ,p5 3突变发生率分别为 64.3 %及 14 .3 %(P <0 .0 5 ) ,高发区组 p5 3突变热点为 2 4 9编码区 ,占该组突变的 77.8%,突变形式为 2 4 9ser突变。结论 :本研究发现高发区组肝癌 p5 3基因突变热点为 2 4 9ser突变 ,应用寡核苷酸芯片技术可并行、高效、多位点检测肿瘤的基因突变更多还原

【Abstract】 Objective:To detect parallel in the pattern of p53 gene point mutation from the paraffin specimen of hepatic cellular cancer(HCC).Methods:We designed oligonucleotide chip which spoted 7 altofrequency mutation sites according to international data base of p53 gene mutation,and detected p53 point mutation by the technique of oligonucleotide chips.Result:The frequency of p53 point mutation from the groups of HCC high and low occurrence rates were 64.3% and 14.3% respectively (P<0.05), and the main mutational form was 249 ser (77.8%, among all mutation) .Conclusion:The point mutational hotspot of p53 gene from HCC high prevalent occur at coding region 249(G T),and oligonucleotide chips can be applied to detect in parallel gene point mutation with high efficiency.更多还原