【摘要】 丽格秋海棠组织培养试验结果表明,利用丽格秋海棠叶片作外植体是较好的取材部位;愈伤组织诱导分化的较佳初始培养基为MS+1mg/L6-BA+0.2mg/L2,4-D+3%白糖+0.4%琼脂或MS+1mg/L6-BA+0.1mg/LNAA+3%白糖+0.4%琼脂;适宜继代增殖培养基为MS+1mg/L6-BA+0.02mg/LNAA+3%白糖+0.4%琼脂;适宜生根培养基为1/2MS+1mg/LNAA+3%白糖+0.4%琼脂;适宜移栽的基质配方为河沙∶珍珠岩=2∶1。移栽适宜温度为18～20℃。更多还原

【Abstract】 The experimental results on tissue culture of Begonia clatior showed that using the leaves as explants was best. The appropriate medium for inducing callus from explants is MS with 1mg/L 6BA + 0.2mg/L 2,4D, 3% sucrose and 0.4% agar, or MS with 1mg/L 6BA + 0.1mg/L NAA+3% sucrose +0.4% agar. The appropriate medium for adventitious buds multiplication is MS with 1mg/L 6BA+0.02mg/L NAA, 3% sucrose and 0.4% agar. The best medium for rooting is 1/2MS with 1mg/L NAA, 3% sucrose and 0.4% agar. The best component ratio of medium for transplanting plants is sand(2) ∶ perlite(1). The best temperature for transplanting is 18~20℃. 更多还原