【摘要】 为研究表皮生长因子(EGF)及表皮生长因子受体(EGFR)在急性放射性皮肤溃疡组织中的表达水平及对溃疡形成、发展、愈合的影响,采用雌性Wistar大鼠,以60Coγ射线局部照射法建立急性放射性皮肤溃疡动物模型,并以手术法建立单纯皮肤伤口动物模型,观察病变55d,采用免疫组化、原位杂交和图像分析等方法检测单纯伤口及皮肤溃疡组织中EGF及其受体的转录和表达水平。结果表明,照射后14d照射野内开始出现皮肤溃疡,之后逐渐扩大、融合、加深。皮肤受照射区多种细胞,特别是溃疡床表皮细胞、成纤维细胞及血管内皮细胞中EGF及其受体的转录及表达水平均较正常皮肤组织有所增强,但与单纯伤口组比较,溃疡组织中EGF及其受体的转录及表达水平明显降低。 说明辐射诱导的皮肤溃疡组织中EGF及其受体表达水平降低可能与溃疡发生、发展及难愈合的分子机制相关。更多还原

【Abstract】 To study EGF and EGFR expression during the formation and development of radiation-induced skin ulcers and their effects on ulcer healing, a rat model which was locally irradiated with 60Co γ-rays was used, and another rat model of simple skin wounds was made as a control. Pathological changes were observed for 55d. Immunohistochemistry in situ hybridization and image analysis were performed to detect EGF and EGFR expression in the tissue of radiation skin ulcers. Results: Skin ulcers were found on 14d after irradiation, and they were enlarged and deepened gradually during the observation period. In the irradiated skin, especially in the epidermal cells, fibroblasts and endothelial cells in the ulcer beds, the expression of EGF and EGFR was higher than that in normal skin. But their expression was obviously decreased in the radiation ulcer beds compared to that in the surgical wound beds. After irradiation, the decreased expression of EGF and EGFR may play an important role in the formation, development and nonhealing mechanism of radiation skin ulcers.更多还原