【摘要】 目的　观察无血清条件下神经酰胺对人肝癌细胞 SMMC- 772 1细胞凋亡的诱导作用。　方法　采用 AO-EB荧光染色、流式细胞术 DNA倍体分析和 DNA琼脂糖凝胶电泳检测细胞凋亡。　结果　终浓度 10 μmol/ L 的 C2 -神经酰胺作用 2 4 h,可诱导无血清培养的 SMMC- 772 1细胞发生凋亡 ,AO- EB染色可见典型的细胞凋亡表现 ,细胞核固缩、浓染、裂解 ,细胞质芽突脱落成为凋亡小体。经流式细胞仪检测可见典型的亚二倍体峰。DNA凝胶电泳呈现典型的 180 - 2 0 0 bp的 DNA梯状条带。　结论　 C2 -神经酰胺可诱导 SMMC- 772 1细胞凋亡。更多还原

【Abstract】 Objective To observe the effect of C\-2\|ceramide on apoptosis of hepatoma cells SMMC\|7721. Methods Microscope, AO\|EB fluorescent staining, flow cytometry and DNA agarose gel electrophoresis were used. Results SMMC\|7721 cells incubated in serum\|free RPMI 1640 medium for 48 h and then treated with 10 μmol/L C\-2\|ceramide for 24 h. C\-2\|ceramide causes cell death in a dose and time\|dependent manner. The apoptotic body and condensation of chromatin were detected with AO\|EB fluorescent staining. The apoptotic peak was seen by the DNA content assay with flow cytometer. DNA agarose gel electrophoresis revealed DNA fragmentation and the appearance of the "DNA ladder". Conclusion C\-2\|ceramide can successfully induce apoptosis of hepatoma cells SMMC\|7721.更多还原