【摘要】 目的　通过免疫组化的方法检测非小细胞肺癌患者术后常规病理检查阴性的淋巴结的微转移灶 ,研究其分子生物学意义。方法　以行肺癌根治性手术的 39名患者为对象 ,用免疫组化角蛋白 (CK )染色的方法检测术后常规病理学检查阴性的 90枚淋巴结中的微转移灶 ,同时也用免疫组化的方法检测相应患者原发肺癌病灶中的 p5 3、p2 1ras和Ki67的表达。结果　 2 2个患者 (5 6.4% )的 2 6枚淋巴结 (2 8.89% )检出微转移灶。原发肺癌灶中有 p5 3、p2 1ras和Ki67表达的患者的淋巴结微转移灶检出率显著高于无这些蛋白表达的患者。肿瘤直径大于 3cm的患者和肿瘤直径小于 3cm的患者的淋巴结微转移灶的检出率分别为 5 5 .6%和5 8.3% ,两者的差别无统计学意义 (P =1.0 0 0 )。结论　原发肺癌病灶中p5 3、p2 1ras和Ki67的表达和淋巴结微转移灶的存在有一定的关系更多还原

【Abstract】 Objective To evaluate the biological significance of the lymphnode micrometastasis in non small cell lung cancer (NSCLC). Methods Ninety regional lymph nodes indicated to be tumor free by conventional histopathologic methods were taken from 39 patients who underwent pulmonary resection for NSCLC. CK immunohistochemical staining was used to detect the micrometastatic tumor cells in lymph nodes. Expressions of p53, p21 ras and Ki67 in primary pulmonary lesions were also detected by immunochemical methods. Results Micrometastasis were found in 26 lymph nodes (28.89%) of 22 patients (56.4%). The proportion of patients with micrometastasis whose primary lesions had the expressions of p53, p21 ras and Ki67 was higher than those without micrometastasis whose primary lesions had no expressions of p53, p21 ras and Ki67. The proportion of patients with micrometastasis whose tumor size was more than and less than 3 cm was 55.6% and 58.3% respectively (P=1.000). Conclusion Expressions of p53, p21 ras and Ki67 in primary pulmonary lesions has certain relations with micrometastasis in lymph nodes.更多还原