【摘要】 目的 :探讨血管内皮生长因子 (vascularendothelialgrowthfactor,VEGF)蛋白质和核酸在卵巢肿瘤组织中的表达与其病理特性和肿瘤转移的关系 ,寻找预后指标和基因治疗的组织细胞 .方法 :恶性卵巢肿瘤 38例、良性卵巢肿瘤 2 7例及正常卵巢组织 2 0例 ,采用SABC免疫组织化学技术检测组织VEGF的蛋白质表达位置及水平 ,原位杂交技术 ,显示组织VEGFmRNA的基因表达和丰度 .结果 :恶性卵巢肿瘤组织VEGF和VEGFmRNA阳性表达率分别显著高于良性肿瘤(P <0 .0 1) ,其二者阳性表达率分别是 89.5 % (34/ 38)和86 .8 % (33/38) ,卵巢癌转移组中二者的表达显著高于未转移组 ,腺癌细胞及周围基质中二者的表达不同步 .结论 :VEGF和VEGFmRNA可以作为卵巢癌侵袭的表型 ,是评估预后的指标 .更多还原

【Abstract】 AIM: To investigate the relationship between the expression of vascular endothelial growth factor (VEGF) protein and mRNA and the pathological properties and metastasis of human ovarian carcinoma. METHODS: The expression of tissue VEGF protein and VEGFmRNA was measured by immunohistochemical and in situ hybridizational technique in 38 cases of ovarian carcinomas and 27 of benign tumors and 20 of normal ovarian. RESULTS: Positive expression rates of VEGF and VEGFmRNA in malignant and benign tissues were 89.5% (34/38) and 86.8% (33/38) respectively and the former level was significantly higher than the latter ( P <0.01). The expression of VEGF and VEGFmRNA was not synchronous. CONCLUSION: Expression of VEGF and VEGFmRNA can be used as an aggressive phenotype and an index of prognostic evaluation in human ovarian carcinoma.更多还原