【摘要】 目的:探讨促肝细胞生长素（pHGF）对大鼠肝星状细胞HSC-T₆增殖及α1（Ⅰ）胶原基因启动子活性的影响。方法:（1）采用MTT法分析pHGF对HSC-T₆细胞增殖的影响。（2）构建含人α1（Ⅰ）胶原基因5′侧翼序列（启动子）与氯霉素乙酰基转移酶（CAT）报告基因的重组体 pCOLH1.5,以脂质体法转染HSC-T₆细胞,ELISA法测定不同浓度pHGF作用24 h后,转染了重组体质粒的HSC-T₆细胞的CAT表达量。结果:（1）不同浓度pHGF对HSC-T₆细胞增殖均有明显的抑制作用,与对照组比较有明显差异（P<0.01）。（2）pHGF在200 μg/ml时对转染重组体质粒pCOLH1.5的HSC-T₆细胞的CAT活性具有明显的抑制作用,与对照组相比差异显著（P<0.05）;增加pHGF浓度至400μg/ml时,对CAT活性抑制更为明显,与对照组相比差异非常显著（P<0.01）。结论:pHGF对HSC-T₆细胞增殖具有明显的抑制作用;对α1（Ⅰ）胶原基因启动子活性具有负性调控作用。更多还原

【Abstract】 Objective: To study the effect of hepatocyte growth-promoting (pHGF) on the proliferation of hepatic stellate cell-T6(HSC-T6)and the activities of α1( Ⅰ) procollagen gene promotor. Methods; (1) Proliferation of HSC-T6 was analyzed by mitochondrial reduction of tetrazolium salt (MTT). (2)The recombinant plasmid pCOLH 1. 5 containing 5’ flank sequence of human α1( Ⅰ ) procollagen gene and chloramphenicol acetyltransf erase (CAT) was transfected into HSC-T6. The activities of CAT were assessed by ELISA method 24 h after pHGF treating. Results: (1) pHGF of different concentrations had inhibiting effects on the proliferation of HSC-T5 vs control group (P<0. 01). (2) pHGF at 200 jug/ml markedly inhibited the activities of CAT in HSC-T6 after transfection of pCOLH1. 5 vs control group (P<0. 05), and when pHGF at 400 μg/ml,the inhibiting effect was more obvious(P<0. 01). Conclusion: pHGF has inhibiting effects on the proliferation of HSC-T6 and negative effects on the activities of α1( Ⅰ ) procollagen gene promotor.更多还原