【摘要】 目的 :探讨 p5 3和 c- myc基因扩增和 m RNA表达在口腔鳞癌 ( OSCC)形成和发展中的作用。方法 :用原位杂交与图像分析相结合的方法 ,对 30例口腔鳞癌和 5例正常口腔粘膜中 p5 3和 c- myc的基因扩增和 m RNA表达状况进行了定性、定位、定量研究。结果 :OSCC中 p5 3和 c- myc的扩增阳性率分别为 40 %和 63.3%,其 m RNA表达率分别是 80 %和 83.3%。p5 3和 c- myc基因扩增量之间具有显著性相关。结论 :这两种基因的扩增和 m RNA表达在 OSCC发生发展中都具有重要作用 ;在 OSCC中导致 p5 3和 c- myc扩增的某些因素可能是相同的更多还原

【Abstract】 Objective:To probe the function of amplification and mRNA expression of p53 and c myc in the formation and development of oral squamous cell carcinomas(OSCC).Methods:Using in situ hybridization and image analysis,the amplification and mRNA expression of p53 and c myc were studied in 30 cases of OSCC and 5 subjects with normal oral mucosa tissues about the nature,location and quantity.Results:The amplification rates of p53 and and c myc were 40% and 63 3% respectively in OSCC,the mRNA expression rates of p53 and c myc were 80% and 83 3% in turn.There was significant correlativity between p53 and c myc in amplification ( P <0 01).Conclustion:The amplification and mRNA expression of p53 and c myc play important roles in the tumorigenesis and development of OSCC.Probably there were some common factors which led p53 and c myc to amplify in OSCC.更多还原