【摘要】 采用含 30mg L蔗糖 ,并附加 5 0 0mg L水解乳蛋白和 4 0mg L腺嘌呤的B5培养基为基本培养基 ,就离体条件下胡杨 (PopuluseuphraticaOliv.)茎段、叶片、嫩根以及茎段愈伤组织不定芽分化增殖途径进行研究 .结果表明 ,维持一定的BA与NAA比值对胡杨不定芽的分化增殖较为重要 ,其中茎段和愈伤组织诱导不定芽以附加 0 5mg LBA、0 1mg LNAA的培养基表现最好 .茎段不定芽再生率及愈伤组织分化增殖率分别为 1 0 0 %和 80 % ;离体叶片以添加0 6mg LBA、0 1mg LNAA的诱导效果最佳 ,诱导率高达 1 0 0 % ,且增殖系数为 1 0 7.离体嫩根则以添加 0 5mg LBA、0 2mg LNAA的处理效果最好 ,诱导率为 35 % .采用繁殖周期、增殖系数、有效芽健壮程度、黄化率等指标 ,对胡杨离体培养 4种分化增殖途径进行比较分析表明 ,胡杨离体茎段培养与叶片诱导不定芽是胡杨试管苗生产的两条良好途径更多还原

【Abstract】 Studies on plantlet in vitro of Populus euphratica Oliver were carried out with the tender shoots from mature trees as the initial explants and B 5 medium as the basic medium, supplemented with 500 mg/L lactalbumin and 40 mg/L laminoparine .The effects of different Plant Growth Regulators (PGRs) on regeneration were compared .The results indicated that the concentrations of PGRs were not strictly required for the excised organs and calli, however the ratio of BA to NAA played the important role . For the adventitious bud regeneration of the stem and callus, supplements with 0 5 mg/L BA and 0 1 mg/L NAA were appropriate, giving a regeneration frequency of 100% and 80%, respectively. The suitable levels of BA and NAA for the excised leaves were 0 6 mg/L and 0 1 mg/L, respectively, yielding a regeneration frequency of 80%. The appropriate levels of BA and NAA for the excised roots were 0 5 mg/L and 0 2 mg/L, obtaining a regeneration frequency of 35%. The differences of regeneration cycle,etiolation rate, bud quality and propagation coefficient among the four proliferation ways were compared and analyzed. The most appropriate two ways for in vitro propagation of Populus euphratica were discussed.更多还原