【摘要】 采用SDS 聚丙烯酰胺凝胶电泳技术 ,对正常和感染粘孢子虫的鲫鱼动脉球、肝脏、鳃、脑、肠、肌肉、眼、鳍 8种组织可溶性蛋白质进行分析比较。结果表明 :病鱼的动脉球、肝脏、鳃、脑、肠 5种组织的蛋白质电泳谱带与正常鱼相比有较大变化 ,动脉球缺失 1条谱带同时又诱导出 1条新谱带 ;肝脏有 9条谱带缺失 ;鳃有 2条谱带缺失的同时诱导出 3条新谱带 ;脑中新增 3条谱带 ;肠中有 5条谱带缺失同时产生 3条新谱带。这些变化可作为辅助疾病诊断的生化指标 ,为从生化水平上揭示该病的致病机理及开展有效的早期防治研究奠定基础。更多还原

【Abstract】 Eight kinds of tissue soluble proteins of normal and Myxosporean-infected C.auratus var. penzesis were analyzed by SDS-polyacrylamid gel electrophoresis (SDS-PAGE). The results showed that the electrophoretic protein bands of artery-ball, liver, gill, brain and intestine of diseased Crucian Carp were different from those of normal ones, with one band disappearing and one new band present in artery-ball; nine bands disappearing in the liver; two bands disappearing and two new bands present in the gill; three new bands present in the brain; five bands disappearing and three new bands present in the intestine. The results indicate that the protein metabolism changes greatly after infection by Myxosporean.The protein changes may be considered as a biochemical diagnosis index and the basis for the study of the pathogenic mechanism as well as for the early prevention of the disease.更多还原