【摘要】 采用PCR技术扩增出猪瘟病毒GXYL株和C株p80基因的NTPase/RNA解旋酶功能区(sGXNS3和sCNS3),将其克隆到表达载体pET-30a(+)中,获得重组质粒pET-sGXNS3和pET-sCNS3。PCR、酶切和序列分析鉴定目的基因插入位置、方向和读码框完全正确。1 0mmol/LIPTG诱导得到分子量为26kD的目的蛋白,Westernblot检测表明,表达的目的蛋白能被CSFV阳性血清识别。更多还原

【Abstract】 Partial NTPase/RNA helicas e functional domain of p80 genes o f CSFV GXYL strain and C strain (s GXNS3 and sCNS3) were amplified by PCRThe recombinant plasmids pET-s GXNS3 and pET-sCNS3 were obtained after being cloned them into expre ssion vector pET-30a(+)The inserted position,the orientation and the O RF were all correct by PCR,restriction enzyme digestion and sequence anal ysisThe 26kD target proteins were produced by inducing with 1mmol/L IPTGWestern-blotting showed that the expressed proteins could be re cognized by CSFV positive serum更多还原