节点文献
海藻多糖的分离纯化和组成分析
Purification and Composition Analysis of a Polysaccharide from Seaweed
【摘要】 从海藻水提取液中得到一种多糖粗提物 ,经DEAE -Cellulose -5 2和Superdex 2 0 0柱层析分离纯化得到均一的多糖组分LJ-S -a ,高效液相色谱法测定其多糖的分子量为 6.8× 10 4 ,气相色谱分析其单糖组成为鼠李糖、葡萄糖和半乳糖 ,其摩尔比依次为 :4.3 0∶1.0 0∶1.88。
【Abstract】 The crude polysaccharide was extracted from Laminaria japonica with hot water and further purification steps that were done through chromatography on DEAE-Cellulose 52 and Superdex 200 column were used to obtain a fraction named LJ-S-a.The fraction was proved to be homogeneous.Its molecular weight was estimated to be 6.8×104 by FPLC. GC analysis indicated that LJ-S-a was composed of Rha ,Glc and Gal in the molar ratio of 4.30∶1.00∶1.88.
【基金】 安徽省教育厅自然科学研究项目 (2 0 0 2KJ0 2 1 )
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2003年05期
- 【分类号】O636.1
- 【被引频次】18
- 【下载频次】1213