【摘要】 目的:探讨芪麝颈康丸对大鼠退变颈椎间盘碱性成纤维细胞生长因子(bFGF)及胰岛素样生长因子(IGF-Ⅰ)mRNA 表达的调节作用。方法:建立动静力失衡性颈椎间盘退变模型,逆转录多聚酶链式反应方法检测椎间盘组织内 bFGF、IGF-ImRNA 表达水平。结果:模型组 bFGF mRNA 表达明显,与正常对照组比较差异有显著性(P<0.01);与模型组比较,芪麝颈康丸、活血通络方、芬必得都可以下调 bFGF,尤以芪麝颈康丸下调明显(P<0.01)。模型组 IGF-Ⅰ mRNA 表达明显减弱,与正常对照组比较差异有显著性(P<0.01);芪麝颈康丸可以上调 IGF-ⅠmRNA(P<0.01),活血通络方、芬必得作用不明显。结论:芪麝颈康丸可以在下调椎间盘组织内 bFGF mRNA,上调 IGF-Ⅰ mRNA 的基因表达。更多还原

【Abstract】 Objective:To study the effects of Qishe Jingkang Pills(QSJKP)on the expression of basic fibrocyte growth factor(bFGF)and insulin-like growth factor-I(IGF-I)mRNA in the degenerated intervertebral disc.Methods:The model of rats degeneration of cervical intervertebral discs caused by imbalance between the dynamic and static force was established.The gene expression of bFGF and IGF-1 mRNA in degeneration cervical intervertebral disc was assayed by reverse transcription polymerase chain reaction(RT-RCR).Results: The expression of bFGF mRNA in the intervertebral disc in model group was markedly higher than that in normal group,and some sig- nificant difference was shown in comparing with QSJKP.The expression of IGF-I mRNA in the intervertebral disc in model group was obviously lower than that in normal group,and significant difference was shown in comparing with QSJKP and model group.Conclu- sion:QSJKP may use to down-regulation gene expressed of bFGF and up-regulation of IGF-I mRNA.更多还原