【摘要】 目的 :探讨重症急性胰腺炎 (ANP)的发病机制及硝普钠的治疗作用。方法 :2 4只大鼠随机分为 3组 :假手术对照组、ANP组和硝普钠 (0 .35 mg/kg)治疗组。采用胰管内注射质量分数为 5 %的牛磺胆酸钠(0 .6 5 ml/kg)的方法建立家兔 ANP模型 ,于制模后 12、2 4和 4 8小时动态观察血浆一氧化氮 (NO)和超氧化物歧化酶 (SOD)的变化及相应胰腺组织学改变。结果 :ANP早期即出现胰腺微循环障碍 ,胰腺组织缺血、缺氧 ,制模后各时间点血浆 NO和 SOD均明显下降 (P均 <0 .0 5 ) ;而硝普钠治疗组血浆 NO、SOD明显高于非治疗组(P均 <0 .0 5 ) ,胰腺组织学改变较 ANP组明显减轻。结论 :氧自由基的生成增加参与了 ANP的病理过程 ,硝普钠的早期应用可以改善胰腺血循环障碍 ,减少氧自由基的生成 ,从而减轻病损改善预后更多还原

【Abstract】 Objective:To investigate the pathogenesis of acute necrotic pancreatitis(ANP) and the effect of sodium nitroprusside(SNP) on ANP induced by sodium taurocholate in rabbits..Methods:Twentyfour rabbits were randomly divided into sham operated group,ANP group,and SNP treatment group(0 35 mg/kg).Plasma levels of nitric oxide(NO) and superoxide dismutase (SOD) were determined at various intervals,and the pathological alterations in pancreatic tissues were also observed.Results:It was found that the pancreatic microcirculation was disturbed at the early stage of ANP and pancreatic tissues were deficient in blood and oxygen delivery.It showed that plasma NO and SOD levels were much lower in ANP group than in the controls (all P <0 05),while they were significantly elevated in SNP treatment group compared to those without treatment (all P <0 05).Meanwhile,the pathological lesions of pancreatic tissues were obviously alleviated in animals treated with SNP.Conclusions:Our data indicate that oxygen free radical(OFR) formation might be involved in the pathogenesis of ANP.Early treatment with SNP can improve pancreatic microcirculation,inhibit OFR release,and attenuats the damage of pancreatic tissues associated with ANP.更多还原